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Published (Version of record)CC BY V4.0, Open Access
Published (Version of record)CC BY V4.0, Open
Journal article
Enriched H3K4me3 marks at Pm-0 resistance-related genes prime courgette against Podosphaera xanthii
Plant Physiology, Vol.188(1), pp.576-592
21/09/2021
PMCID: PMC8774738
PMID: 34597395
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Source: InCites
Abstract
Powdery mildew (PM) disease, caused by the obligate biotrophic fungal pathogen Podosphaera xanthii, is the most reported and destructive disease on cultivated Cucurbita species all over the world. Recently, the appearance of highly aggressive P. xanthii isolates has led to PM outbreaks even in resistant crops, making disease management a very difficult task. To challenge this, breeders rely on genetic characteristics for PM control. Analysis of commercially available intermediate resistance courgette (Cucurbita pepo L. var. cylindrica) varieties using cytological, molecular, and biochemical approaches showed that the plants were under a primed state and induced systemic acquired resistance (SAR) responses, exhibiting enhanced callose production, upregulation of salicylic acid (SA) defense signaling pathway genes, and accumulation of SA and defense metabolites. Additionally, the intermediate resistant varieties showed an altered epigenetic landscape in histone marks that affect transcriptional activation. We demonstrated that courgette plants had enriched H3K4me3 marks on SA-BINDING PROTEIN 2 and YODA (YDA) genes of the Pm-0 interval introgression, a genomic region that confers resistant to Cucurbits against P. xanthii. The open chromatin of SA-BINDING PROTEIN 2 and YDA genes was consistent with genes’ differential expression, induced SA pathway, altered stomata characteristics, and activated SAR responses. These findings demonstrate that the altered epigenetic landscape of the intermediate resistant varieties modulates the activation of SA-BINDING PROTEIN 2 and YDA genes leading to induced gene transcription that primes courgette plants.
Details
- Title
- Enriched H3K4me3 marks at Pm-0 resistance-related genes prime courgette against Podosphaera xanthii
- Creators
- Theoni Margaritopoulou - Benaki Phytopathological InstituteDimosthenis Kizis - Benaki Phytopathological InstituteDimitris Kotopoulis - Benaki Phytopathological InstituteIoannis E. Papadakis - Agricultural University of AthensChristos Anagnostopoulos - Benaki Phytopathological InstituteEirini Baira - Benaki Phytopathological InstituteAikaterini Termentzi - Benaki Phytopathological InstituteAikaterini-Eleni Vichou - Benaki Phytopathological InstituteCarlo Leifert - Southern Cross UniversityEmilia Markellou - Benaki Phytopathological Institute
- Publication Details
- Plant Physiology, Vol.188(1), pp.576-592
- Publisher
- American Society of Plant Biologists
- Number of pages
- 1
- Grant note
- We acknowledge the support of this work by the project “PlantUp” (MIS 5002803) which is implemented under the Action “Reinforcement of the Research and Innovation Infrastructure,” funded by the Operational Programme “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014-2020) and co-financed by Greece and the European Union (European Regional Development Fund), and by the funds from Benaki Phytopathological Institute.
- Identifiers
- 991013055106502368
- Copyright
- © The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
- Academic Unit
- Southern Cross Plant Science; Faculty of Science and Engineering
- Language
- English
- Resource Type
- Journal article