Journal article
Endometrial biopsy in Bos indicus beef heifers
Reproduction in domestic animals, Vol.52(3), pp.526-528
06/2017
PMID: 28295721
Metrics
7 Record Views
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Abstract
An endometrial biopsy allows for a comprehensive assessment of the uterine environment of a breeding female. Although routine in mares, devices used for endometrial biopsies are impracticable in heifers due to the size and structure of the cervix. This report describes the use of a human bronchoscopy biopsy device (Karl Storz® 10366L) for collection of endometrial biopsies in Bos indicus beef heifers. The Storz® device is smaller and thinner and enabled the collection of an endometrial biopsy in 86% of heifers (n = 44/51). The biopsied tissue was of good quality and suitable for transcriptomic assessment of the endometrium, with total RNA yield and RNA integrity number (RIN) averaging 1.3 μg (range 0.4–5.3 μg) and 7.4 (range 5.7–8.4), respectively.
Details
- Title
- Endometrial biopsy in Bos indicus beef heifers
- Creators
- O Ramirez-Garzon - University of QueenslandN Satake - University of QueenslandR E Lyons - University of QueenslandJ Hill - University of QueenslandM K Holland - University of QueenslandM McGowan - University of Queensland
- Publication Details
- Reproduction in domestic animals, Vol.52(3), pp.526-528
- Publisher
- Wiley-Blackwell Verlag GmbH
- Grant note
- Departamento Administrativo de Ciencia, Tecnología e Innovación. Grant Number: 568, 2012 School of Veterinary Science The University of Queensland, Australia
- Identifiers
- 991013131701902368
- Copyright
- © 2017 Blackwell Verlag GmbH.
- Academic Unit
- Office of the Vice Chancellor
- Language
- English
- Resource Type
- Journal article