Journal article
Effects of dilute acid pretreatment on enzyme saccharification of wheat stubble
Journal of Chemical Technology and Biotechnology, Vol.86(6), pp.818-825
06/2011
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Abstract
BACKGROUND: Prehydrolysis of wheat stubble using moderate temperatures and dilute acid strength is an effective means for solubilizing hemicellulose fractions and improving cellulose hydrolysis. Variation in prehydrolysis parameters (temperature, time, and acid strength) and enzymatic saccharification conditions were examined for conversion of wheat stubble into fermentable sugars.
RESULTS: Elevating temperature and acid strength maximized sugar release in prehydrolysate liquors. The optimal conditions of 2.0% H2SO4/60 min/121 ◦ C effectively solubilized 79% of the available hemicellulose. Production of inhibitory hydrolysis and degradation products such as acetic acid and levulinic acid, were detected at levels of 3.4 g L−1 and 0.64 g L−1, respectively. Sugar yields in prehydrolysate and saccharified liquors were found to increase with treatment severity. Temperature had the greatest impact on sugar release, followed by acid concentration and time. Optimizing prehydrolysis conditions at 1.0% H2SO4/90 min/121 ◦ C, produced a 3.2-fold improvement in cellulose hydrolysis with recoveries approaching 82%. The addition of β-glucosidase and xylanase to the cellulase preparations assisted monomeric sugar release.
Details
- Title
- Effects of dilute acid pretreatment on enzyme saccharification of wheat stubble
- Creators
- Tony Vancov - NSW Department of Primary IndustriesShane McIntosh - NSW Department of Primary Industries
- Publication Details
- Journal of Chemical Technology and Biotechnology, Vol.86(6), pp.818-825
- Publisher
- John Wiley & Sons, Ltd
- Number of pages
- 8
- Identifiers
- 991013015697902368
- Academic Unit
- Faculty of Science and Engineering
- Language
- English
- Resource Type
- Journal article