Journal article
Effects of brewing conditions on the antioxidant capacity of twenty-four commercial green tea varieties
Food chemistry, Vol.192, pp.380-387
01/02/2016
PMCID: PMC4549831
PMID: 26304363
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Source: InCites
Abstract
A novel paper-based Nanoceria Reducing Antioxidant Capacity (NanoCerac) assay for antioxidant detection (Sharpe, Frasco, Andreescu, & Andreescu, 2012), has been adapted for the first time as a high-throughput method, in order to measure the effect of brewing conditions and re-infusion on the antioxidant capacity of twenty-four commercial green teas. The oxygen radical absorbance capacity (ORAC) assay, frequently applied to complex foods and beverages, was used as a comparator measure of antioxidant capacity. A novel measure of sustained antioxidant capacity, the total inherent antioxidant capacity (TI-NanoCerac and TI-ORAC) was measured by infusing each tea six times. Effects of brewing conditions (temperature, brew time, etc.) were assessed using one popular tea as a standard. Both NanoCerac and ORAC assays correlated moderately (R2 0.80 ± 0.19). The average first-brew NanoCerac, TI-NanoCerac, first-brew ORAC and TI-ORAC were: 0.73 ± 0.1 GAE/g tea; 2.4 ± 0.70 mmol GAE/g tea; 1.0 ± 0.3 mmol TE/g tea and 2.1 ± 0.71 mmol TE/g tea respectively. Brewing conditions including water temperature and infusion time significantly affected antioxidant capacity. The high-throughput adaptation of the original NanoCerac assay tested here offered advantages over ORAC, including portability and rapid analysis.
Details
- Title
- Effects of brewing conditions on the antioxidant capacity of twenty-four commercial green tea varieties
- Creators
- Erica Sharpe - SUNY CantonFang Hua - Clarkson UniversityStephanie Schuckers - Clarkson UniversitySilvana Andreescu - Clarkson UniversityRyan Bradley - National University of Natural Medicine
- Publication Details
- Food chemistry, Vol.192, pp.380-387
- Publisher
- Elsevier Ltd
- Identifiers
- 991013036337902368
- Academic Unit
- National Centre for Naturopathic Medicine
- Language
- English
- Resource Type
- Journal article