Journal article
Effects and potential mechanisms of Danzhi Xiaoyao Pill on proliferation of MCF-7 human breast cancer cells in vitro
Chinese Journal of Integrative Medicine, Vol.14(2), pp.128-131
2008
Metrics
24 Record Views
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Abstract
<p>Objective: To investigate the effects of 50% ethyl alcohol (EtOH) extracts from Danzhi Xiaoyao Pill (DXP) on the proliferation of MCF-7 human breast cancer cells and potential mechanisms. Methods: ATP-Lite assay was performed to test the proliferation of the MCF-7 breast cancer cell line; and antioxidant activity was measured by the oxygen radical absorbance capacity (ORAC). The effects of DXP on nitric oxide (NO) production were tested by lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophages using the Griess reaction. Results: The 50% EtOH DXP extracts displayed a cytotoxic response on MCF-7 cells at 0.10, 0.25 and 0.50 mg/mL dose-dependently with the proliferation inhibited by more than 85%. The ORAC value of the DXP was 820 μ moL Trolox equivalent/g, about 40% of the vitamin C value. DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL (P<0.05, P<0.01). Conclusion: The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells, with the effect possibly related to its antioxidant activity and the inhibition of NO production.</p>
Details
- Title
- Effects and potential mechanisms of Danzhi Xiaoyao Pill on proliferation of MCF-7 human breast cancer cells in vitro
- Creators
- Hui Liao - Southern Cross UniversityLinda K Banbury - Southern Cross UniversityDavid N Leach - Southern Cross University
- Publication Details
- Chinese Journal of Integrative Medicine, Vol.14(2), pp.128-131
- Identifiers
- 1040; 991012820705502368
- Academic Unit
- Southern Cross Plant Science
- Resource Type
- Journal article