Journal article
Effect of α-Amylase, Papain, and Spermfluid® treatments on viscosity and semen parameters of dromedary camel ejaculates
Research in veterinary science, Vol.105, pp.5-9
01/04/2016
PMID: 27033899
Metrics
1 Record Views
Abstract
Ejaculates from five clinically healthy dromedary camels (Camelus dromedarius) were used to evaluate the effects of different enzymatic treatments (Amylase, Papain, Spermfluid®) on liquefaction and seminal parameters. After collection, ejaculates were divided into 5 aliquots: (1) kept undiluted (control); or diluted 1:1 with: (2) Tris-Citrate-Fructose (TCF), (3) TCF containing Amylase, (4) TCF containing Papain or (5) Spermfluid® containing Bromelain. At 120min after dilution, each aliquot was evaluated, at 20-min intervals, for viscosity, motility, viability and agglutination. Only the aliquots diluted with TCF containing Papain underwent complete liquefaction. Sperm motility decreased significantly during the observation times, except for the samples diluted with Spermfluid® (P=0.005). Diluted samples showed different levels of agglutination, with the lowest being observed in the control and the highest in the Papain-treated samples. The viscosity of dromedary camel ejaculates could be effectively reduced by using the proteolytic enzyme Papain.
Details
- Title
- Effect of α-Amylase, Papain, and Spermfluid® treatments on viscosity and semen parameters of dromedary camel ejaculates
- Creators
- Davide Monaco - University of Bari Aldo MoroMeriem Fatnassi - University of GabèsBarbara Padalino - University of Bari Aldo MoroMohamed Hammadi - University of GabèsTouhami Khorchani - University of GabèsGiovanni Michele Lacalandra - University of Bari Aldo Moro
- Publication Details
- Research in veterinary science, Vol.105, pp.5-9
- Publisher
- Elsevier Ltd
- Number of pages
- 5
- Grant note
- This document has been produced with the financial assistance of the European Union through the “PROCAMED” Project: Promotion des systèmes camelins innovants et des filières locales pour une gestion durable des territoires sahéliens: reference number: I.B/1.1/493.
- Identifiers
- 991013293554002368
- Copyright
- © 2016 Elsevier Ltd. All rights reserved.
- Academic Unit
- Faculty of Science and Engineering; Veterinary Science
- Language
- English
- Resource Type
- Journal article