Effect of temperature and colonization of Legionella pneumophila and Vermamoeba vermiformis on bacterial community composition of copper drinking water biofilms

Helen Y. Buse; Pan Ji; Vicente Gomez-Alvarez; Amy Pruden; Marc A. Edwards; Nicholas J. Ashbolt

doi:10.1111/1751-7915.12457

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Effect of temperature and colonization of Legionella pneumophila and Vermamoeba vermiformis on bacterial community composition of copper drinking water biofilms

Journal article

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Effect of temperature and colonization of Legionella pneumophila and Vermamoeba vermiformis on bacterial community composition of copper drinking water biofilms

Helen Y. Buse, Pan Ji, Vicente Gomez-Alvarez, Amy Pruden, Marc A. Edwards and Nicholas J. Ashbolt

Microbial biotechnology, Vol.10(4), pp.773-788

07/2017

DOI: https://doi.org/10.1111/1751-7915.12457

PMCID: PMC5481522

PMID: 28097816

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Abstract

Biotechnology & Applied Microbiology

Life Sciences & Biomedicine

Microbiology

Science & Technology

It is unclear how the water-based pathogen, Legionella pneumophila (Lp), and associated free-living amoeba (FLA) hosts change or are changed by the microbial composition of drinking water (DW) biofilm communities. Thus, this study characterized the bacterial community structure over a 7-month period within mature (> 600-day-old) copper DW biofilms in reactors simulating premise plumbing and assessed the impact of temperature and introduction of Lp and its FLA host, Vermamoeba vermiformis (Vv), co-cultures (LpVv). Sequence and quantitative PCR (qPCR) analyses indicated a correlation between LpVv introduction and increases in Legionella spp. levels at room temperature (RT), while at 37°C, Lp became the dominant Legionella spp. qPCR analysis suggested Vv presence may not be directly associated with Lp biofilm growth at RT and 37°C, but may contribute to or be associated with non-Lp legionellae persistence at RT. Two-way PERMANOVA and PCoA revealed that temperature was a major driver of microbiome diversity. Biofilm community composition also changed over the seven-month period and could be associated with significant shifts in dissolved oxygen, alkalinity and various metals in the influent DW. Hence, temperature, biofilm age, DW quality and transient intrusions/amplification of pathogens and FLA hosts may significantly impact biofilm microbiomes and modulate pathogen levels over extended periods.

Details

Title: Effect of temperature and colonization of Legionella pneumophila and Vermamoeba vermiformis on bacterial community composition of copper drinking water biofilms
Creators: Helen Y. Buse
Pan Ji - Virginia Tech
Vicente Gomez-Alvarez
Amy Pruden - Virginia Tech
Marc A. Edwards - Virginia Tech
Nicholas J. Ashbolt - University of Alberta
Publication Details: Microbial biotechnology, Vol.10(4), pp.773-788
Publisher: Wiley
Number of pages: 16
Grant note: 1336650 / National Science Foundation CBET award; National Science Foundation (NSF) Alfred P. Sloan Foundation Microbiology of the Built Environment programme 201300490 / Alberta Innovates Alberta Innovates-Health Solutions Virginia Tech Institute for Critical Technology and Applied Science
Identifiers: 991013097815102368
Copyright: © 2016 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution License, (https://creativecommons.org/licenses/by/4.0/).
Academic Unit: Faculty of Science and Engineering
Language: English
Resource Type: Journal article

Effect of temperature and colonization of Legionella pneumophila and Vermamoeba vermiformis on bacterial community composition of copper drinking water biofilms

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