Journal article
Effect of Disinfectant Exposure on Legionella pneumophila Associated with Simulated Drinking Water Biofilms: Release, Inactivation, and Infectivity
Environmental science & technology, Vol.51(4), pp.2087-2095
21/02/2017
PMID: 28085262
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Source: InCites
Abstract
Legionella pneumophila, the most commonly identified causative agent in drinking water associated with disease outbreaks, can be harbored by and released from drinking water biofilms. In this study, the release of biofilm-associated L. pneumophila under simulated drinking water flow containing a disinfectant residual was examined. Meanwhile, the inactivation and infectivity (to amoebae) of the released L. pneumophila were studied. To simulate drinking water system conditions, biofilms were prepared under either disinfectant exposure (predisinfected biofilms) or disinfectant-free (untreated biofilms) conditions, respectively. For experiments with water flow containing a disinfectant to release the biofilm-associated L. pneumophila from these two types of biofilms, the L. pneumophila release kinetics values from predisinfected and untreated biofilms under flow condition were not statistically different (one-way ANOVA, p > 0.05). However, inactivation of the L. pneumophila released from predisinfected biofilms was 1-2 times higher and amoeba infectivity was 2-29 times lower than that from untreated biofilms. The higher disinfectant resistance of L. pneumophila released from untreated biofilms was presumably influenced by the detachment of a larger amount of biofilm material (determined by 16S rRNA qPCR) surrounding the released L. pneumophila. This study highlights the interaction among disinfectant residual, biofilms, and L. pneumophila, which provides guidelines to assess and control pathogen risk.
Details
- Title
- Effect of Disinfectant Exposure on Legionella pneumophila Associated with Simulated Drinking Water Biofilms: Release, Inactivation, and Infectivity
- Creators
- Yun Shen - University of Illinois Urbana-ChampaignConghui Huang - University of Illinois at Urbana ChampaignJie Lin - University of Illinois at Urbana ChampaignWenjing Wu - University of Illinois Urbana-ChampaignNicholas J Ashbolt - University of AlbertaWen-Tso Liu - University of Illinois at Urbana ChampaignThanh H Nguyen - University of Illinois at Urbana Champaign
- Publication Details
- Environmental science & technology, Vol.51(4), pp.2087-2095
- Publisher
- American Chemical Society
- Identifiers
- 991013097816502368
- Copyright
- Copyright © 2017 American Chemical Society.
- Academic Unit
- Faculty of Science and Engineering
- Language
- English
- Resource Type
- Journal article