Dissecting the relationship between plasma and tissue metabolome in a cohort of women with obesity: Analysis of subcutaneous and visceral adipose, muscle, and liver

Zhanxuan E Wu; Marlena C Kruger; Garth J S Cooper; Ivana R Sequeira; Anne-Thea McGill; Sally D Poppitt; Karl Fraser

doi:10.1096/fj.202101812R

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Dissecting the relationship between plasma and tissue metabolome in a cohort of women with obesity: Analysis of subcutaneous and visceral adipose, muscle, and liver

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Dissecting the relationship between plasma and tissue metabolome in a cohort of women with obesity: Analysis of subcutaneous and visceral adipose, muscle, and liver

Zhanxuan E Wu, Marlena C Kruger, Garth J S Cooper, Ivana R Sequeira, Anne-Thea McGill, Sally D Poppitt and Karl Fraser

The FASEB Journal, Vol.36(7), e22371

07/2022

DOI: https://doi.org/10.1096/fj.202101812R

PMID: 35704337

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Abstract

tissue metabolomics

clinical metabolomics

liquid chromatography-mass spectrometry

biomarkers

Untargeted metabolomics of blood samples has become widely applied to study metabolic alterations underpinning disease and to identify biomarkers. However, understanding the relevance of a blood metabolite marker can be challenging if it is unknown whether it reflects the concentration in relevant tissues. To explore this field, metabolomic and lipidomic profiles of plasma, four sites of adipose tissues (ATs) from peripheral or central depot, two sites of muscle tissue, and liver tissue from a group of nondiabetic women with obesity who were scheduled to undergo bariatric surgery (n = 21) or other upper GI surgery (n = 5), were measured by liquid chromatography coupled with mass spectrometry. Relationships between plasma and tissue profiles were examined using Pearson correlation analysis subject to Benjamini-Hochberg correction. Plasma metabolites and lipids showed the highest number of significantly positive correlations with their corresponding concentrations in liver tissue, including lipid species of ceramide, mono- and di-hexosylceramide, sphingomyelin, phosphatidylcholine (PC), phosphatidylethanolamine (PE), lysophosphatidylethanolamine, dimethyl phosphatidylethanolamine, ether-linked PC, ether-linked PE, free fatty acid, cholesteryl ester, diacylglycerol and triacylglycerol, and polar metabolites linked to several metabolic functions and gut microbial metabolism. Plasma also showed significantly positive correlations with muscle for several phospholipid species and polar metabolites linked to metabolic functions and gut microbial metabolism, and with AT for several triacylglycerol species. In conclusion, plasma metabolomic and lipidomic profiles were reflective more of the liver profile than any of the muscle or AT sites examined in the present study. Our findings highlighted the importance of taking into consideration the metabolomic relationship of various tissues with plasma when postulating plasma metabolites marker to underlying mechanisms occurring in a specific tissue.

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Title: Dissecting the relationship between plasma and tissue metabolome in a cohort of women with obesity: Analysis of subcutaneous and visceral adipose, muscle, and liver
Creators: Zhanxuan E Wu - High-Value Nutrition National Science Challenge, Auckland, New Zealand
Marlena C Kruger - Riddet Institute, Massey University, Palmerston North, New Zealand
Garth J S Cooper - University of Manchester
Ivana R Sequeira - Human Nutrition Unit, School of Biological Sciences, University of Auckland, Auckland, New Zealand
Anne-Thea McGill - Southern Cross University
Sally D Poppitt - University of Auckland
Karl Fraser - Riddet Institute, Massey University, Palmerston North, New Zealand
Publication Details: The FASEB Journal, Vol.36(7), e22371
Publisher: John Wiley & Sons, Inc.
Grant note: 08/190C / Manatu Hauora | Health Research Council of New Zealand (HRC) 3710040 / Ministry for Business Innovation and Employment (MBIE)
Identifiers: 991013032083902368
Copyright: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2022 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology
Academic Unit: Faculty of Health; National Centre for Naturopathic Medicine
Language: English
Resource Type: Journal article

Dissecting the relationship between plasma and tissue metabolome in a cohort of women with obesity: Analysis of subcutaneous and visceral adipose, muscle, and liver

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