Journal article
Discovery of a New Source of Rifamycin Antibiotics in Marine Sponge Actinobacteria by Phylogenetic Prediction
Applied and environmental microbiology, Vol.72(3), pp.2118-2125
2006
PMCID: PMC1393243
PMID: 16517661
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Source: InCites
Abstract
Phylogenetic analysis of the ketosynthase (KS) gene sequences of marine sponge-derived Salinispora strains of actinobacteria indicated that the polyketide synthase (PKS) gene sequence most closely related to that of Salinispora was the rifamycin B synthase of Amycolatopsis mediterranei. This result was not expected from taxonomic species tree phylogenetics using 16S rRNA sequences. From the PKS sequence data generated from our sponge-derived Salinispora strains, we predicted that such strains might synthesize rifamycin-like compounds. Liquid chromatography-tandem mass spectrometry (LC/MS/MS) analysis was applied to one sponge-derived Salinispora strain to test the hypothesis of rifamycin synthesis. The analysis reported here demonstrates that this Salinispora isolate does produce compounds of the rifamycin class, including rifamycin B and rifamycin SV. A rifamycin-specific KS primer set was designed, and that primer set increased the number of rifamycin-positive strains detected by PCR screening relative to the number detectable using a conserved KS-specific set. Thus, the Salinispora group of actinobacteria represents a potential new source of rifamycins outside the genus Amycolatopsis and the first recorded source of rifamycins from marine bacteria.
Details
- Title
- Discovery of a New Source of Rifamycin Antibiotics in Marine Sponge Actinobacteria by Phylogenetic Prediction
- Creators
- Tae Kyung Kim - University of QueenslandAmitha K Hewavitharana - University of QueenslandP. Nicholas Shaw - University of QueenslandJohn A Fuerst (Corresponding Author) - University of Queensland
- Publication Details
- Applied and environmental microbiology, Vol.72(3), pp.2118-2125
- Publisher
- American Society for Microbiology
- Identifiers
- 991012893699102368
- Academic Unit
- Faculty of Science and Engineering; Southern Cross Plant Science; Science
- Language
- English
- Resource Type
- Journal article