Journal article
Cyclosporine A Modulates Neuroinflammation-Related Gene Expression Associated with Alzheimer’s Disease in SH-SY5Y Neuronal Cell Line: Is Cyclosporine A Beneficial/Detrimental?
Biochemical genetics, Vol.64(3), pp.3682-3704
06/2026
PMID: 40750957
Appears in Recent Faculty of Health Publications
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Abstract
Numerous investigations indicate that cyclosporine A (CsA) significantly influences the prevention or progression of Alzheimer’s disease (AD) through various mechanisms. The precise pathways by which CsA, an inhibitor of calcineurin (CN) and peptidyl-prolyl isomerase A (PPIA), operates remain incompletely elucidated. In this study, we explored the effects of CsA, a widely utilized immunosuppressive agent, on the gene expression of various factors related to inflammation, cell adhesion, intercellular communication, and apoptosis in SH-SY5Y cells. We employed the semi-quantitative real-time PCR (RT-qPCR) method for gene expression analysis to assess these effects. Furthermore, network pharmacology was utilized to identify the potential targets of CsA and to further analyze. Our results demonstrated that CsA enhances the mRNA levels of most factors examined, including IL1B, S100A9, CD147, TREM2, LRP1, MAPK1, MAPK14, GSK3B, Dynamin 1, Dynamin 3, NLRP3, NLRP1, CASPASE 3, CASPASE 1, AIFM1, and STAT3. At the same time, it suppresses the mRNA levels of PPIA, TLR2, TLR4, PYCARD, RAGE, and BCL2. The network pharmacology analysis revealed seven target genes overlapping our experimental findings (PPIA, S100A9, TLR4, STAT3, MAPK1, MAPK14, and BAX). Our results suggest that CsA modulates gene expression in SH-SY5Y cells, with the potential for either beneficial/ harmful effects on cellular function, depending on the specific roles of the assessed genes.
Details
- Title
- Cyclosporine A Modulates Neuroinflammation-Related Gene Expression Associated with Alzheimer’s Disease in SH-SY5Y Neuronal Cell Line: Is Cyclosporine A Beneficial/Detrimental?
- Creators
- Somayeh Pashaei - Kermanshah University of Medical SciencesLudmilla A. Morozova-Roche - Umeå UniversityZohreh Rahimi - Kermanshah University of Medical SciencesSoheila Mohammadi - Kermanshah University of Medical SciencesEbrahim Barzegari - Kermanshah University of Medical SciencesSasan Shabani - Kermanshah University of Medical SciencesNader Salari - Kermanshah University of Medical SciencesReza Khodarahmi - Kermanshah University of Medical Sciences
- Publication Details
- Biochemical genetics, Vol.64(3), pp.3682-3704
- Publisher
- Springer Nature
- Number of pages
- 23
- Grant note
- The authors express their sincere gratitude to the research council of Kermanshah University of Medical Sciences for their financial support (Grant Number 4000930) and to the Iran National Science Foundation (Grant Number 4026116).
- Identifiers
- 991013400936302368
- Copyright
- © 2025, The Author(s), under exclusive licence to Springer Science Business Media, LLC, part of Springer Nature
- Academic Unit
- Faculty of Health
- Language
- English
- Resource Type
- Journal article