Comparative transcriptomic analysis of wheat cultivars differing in their resistance to Fusarium head blight infection during grain-filling stages reveals unique defense mechanisms at play

Can Chen; Qi Guo; Qifang He; Zhuangbo Tian; Weihao Hao; Xinyu Shan; Jie Lu; Bronwyn J Barkla; Chuanxi Ma; Hongqi Si

doi:10.1186/s12870-023-04451-0

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Comparative transcriptomic analysis of wheat cultivars differing in their resistance to Fusarium head blight infection during grain-filling stages reveals unique defense mechanisms at play

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Comparative transcriptomic analysis of wheat cultivars differing in their resistance to Fusarium head blight infection during grain-filling stages reveals unique defense mechanisms at play

Can Chen, Qi Guo, Qifang He, Zhuangbo Tian, Weihao Hao, Xinyu Shan, Jie Lu, Bronwyn J Barkla, Chuanxi Ma and Hongqi Si

BMC plant biology, Vol.23, 433

16/09/2023

DOI: https://doi.org/10.1186/s12870-023-04451-0

PMID: 37715120

Appears in Recent Faculty of Science and Engineering Publications

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Abstract

Pathogen defence

Immune response

Toxin accumulation

Cell wall

Biotic stress

Wheat head blight

Stress resistance

Fusarium head blight (FHB) is a devastating fungal disease that poses a significant threat to wheat production, causing substantial yield losses. Understanding the molecular mechanisms of wheat resistance to FHB is crucial for developing effective disease management strategies. This study aimed to investigate the mechanisms of FHB resistance and the patterns of toxin accumulation in three wheat cultivars, Annong8455, Annong1589, and Sumai3, with different levels of resistance, ranging from low to high respectively, under natural field conditions. Samples were taken at three different grain-filling stages (5, 10, and 15 DPA) for gene expression analysis and phenotypic observation. Results found that toxin concentration was inversely correlated with varietal resistance but not correlated with disease phenotypes, indicating that toxin analysis is a more accurate measure of disease status in wheat ears and grains. Transcriptomic data showed that Sumai3 exhibited a stronger immune response during all stages of grain filling by upregulating genes involved in the active destruction of pathogens and removal of toxins. In contrast, Annong1589 showed a passive prevention of the spread of toxins into cells by the upregulation of genes involved in tyramine biosynthesis at the early stage (5 DPA), which may be involved in cell wall strengthening. Our study demonstrates the complexity of FHB resistance in wheat, with cultivars exhibiting unique and overlapping defence mechanisms, and highlights the importance of considering the temporal and spatial dynamics of gene expression in breeding programs for developing more resistant wheat cultivars.

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Title: Comparative transcriptomic analysis of wheat cultivars differing in their resistance to Fusarium head blight infection during grain-filling stages reveals unique defense mechanisms at play
Creators: Can Chen - Anhui Agricultural University
Qi Guo - Southern Cross University
Qifang He - Anhui Agricultural University
Zhuangbo Tian - Anhui Agricultural University
Weihao Hao - Anhui Agricultural University
Xinyu Shan - Anhui Agricultural University
Jie Lu - Anhui Agricultural University
Bronwyn J Barkla - Southern Cross University
Chuanxi Ma - Anhui Agricultural University
Hongqi Si - Anhui Agricultural University
Publication Details: BMC plant biology, Vol.23, 433
Publisher: BioMed Central
Grant note: This research was funded by Grants from the Natural Science Research Project of Anhui Educational Committee (2022AH050887), the Agriculture Research System (CARS-03), the key scientific and technological breakthroughs of Anhui Province (2021d06050003), Anhui Provincial and Ministerial Collaborative Innovation Center for Food Crops (APMCIC-FC), and Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP).
Identifiers: 991013140913702368
Academic Unit: Southern Cross Analytical Research Services; Science; Faculty of Science and Engineering
Language: English
Resource Type: Journal article

Comparative transcriptomic analysis of wheat cultivars differing in their resistance to Fusarium head blight infection during grain-filling stages reveals unique defense mechanisms at play

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