Journal article
Characterization of Ice-Binding Proteins from Sea-Ice Microalgae
Methods in Molecular Biology, Vol.2156, pp.289-302
2020
PMID: 32607989
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Abstract
Several species of polar microalgae are able to live and thrive in the extreme environment found within sea ice, where ice crystals may reduce the organisms’ living space and cause mechanical damage to the cells. Among the strategies adopted by these organisms to cope with the harsh conditions in their environment, ice-binding proteins (IBPs) seem to play a key role and possibly contribute to the success of microalgae in sea ice. Indeed, IBPs from microalgae predominantly belong to the so-called “DUF 3494-IBP” family, which today represents the most widespread IBP family. Since IBPs have the ability to control ice crystal growth, their mechanism of function is of interest for many potential applications. Here, we describe methods for a classical determination of the IBP activity (thermal hysteresis, recrystallization inhibition) and further methods for protein activity characterization (ice pitting assay, determination of the nucleating temperature).
Details
- Title
- Characterization of Ice-Binding Proteins from Sea-Ice Microalgae
- Creators
- Maddalena Bayer-Giraldi - Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, The (AWI)EonSeon Jin - Hanyang UniversityPeter W Wilson - Southern Cross University
- Publication Details
- Methods in Molecular Biology, Vol.2156, pp.289-302
- Publisher
- Humana Press, Inc.; United States
- Identifiers
- 991012925097102368
- Academic Unit
- Faculty of Science and Engineering; Science; School of Environment, Science and Engineering
- Language
- English
- Resource Type
- Journal article