Journal article
Characterisation of Sweet potato collusive virus (SPCV) isolates from sweet potato (Ipomea batatas) in Australia
Australasian plant pathology, Vol.51(4), pp.391-397
01/07/2022
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Abstract
Sweet potato collusive virus (SPCV) is a member of the genus Cavemovirus, family Caulimoviridae, for which only one full-length genome sequence has been reported. SPCV was first detected in Australia in 2007 in two sweet potato accessions using the nitrocellulose membrane ELISA kit developed by the International Potato Centre (CIP). Infected plants were also shown to contain isometric virions of similar to 50 nm, typical of members of the genera Cavemovirus, Caulimovirus, Petuvirus and Soymovirus. We have now sequenced and characterised the complete genomes of the two SPCV isolates (designated SPCV-Aus1 and -Aus2) using a combination of next-generation sequencing and PCR/Sanger sequencing. The sequences of both isolates encode three major ORFs with a genome organisation typical of cavemoviruses. However, isolate SPCV-Aus2 possesses a considerably shorter genome length of 7275 bp compared to SPCV-Aus1 (7712 bp) and the only available full-length sequence from a Portuguese isolate (7723 bp; GenBank accession number NC_015328). Further, ORF 1 of SPCV-Aus2 is considerably shorter than the ORF 1 length of both SPCV-Aus1 and SPCV-Mad1. Phylogenetic and PASC analysis showed that SPCV-Aus1 is closely related to SPCV isolates from North and Central America, whereas SPCV-Aus2 clustered together with isolates from Portugal and Africa.
Details
- Title
- Characterisation of Sweet potato collusive virus (SPCV) isolates from sweet potato (Ipomea batatas) in Australia
- Creators
- Amit C. Sukal - Queensland University of TechnologySandra Dennien - Department of Primary IndustriesDawit B. Kidanemariam - Queensland University of TechnologyKarlah Norkunas - Queensland University of TechnologyEric Coleman - Central Queensland UniversityRobert M. Harding - Queensland University of TechnologyAnthony P. James - Queensland University of Technology
- Publication Details
- Australasian plant pathology, Vol.51(4), pp.391-397
- Publisher
- Springer Nature
- Number of pages
- 7
- Grant note
- Australian Sweet Potato Grower's Inc Australian Sweet Potato Farmers VG13004 / Australian Sweet Potato Grower's Inc./Hort. Innovation Australia SCMN/2004/071; CP2005/134; PC2010/026 / Australian Centre for International Agricultural Research (ACIAR); Australian Centre for International Agricultural Research DAF
- Identifiers
- 991013372729402368
- Academic Unit
- Faculty of Health
- Language
- English
- Resource Type
- Journal article