Journal article
Assessing medicinal plants from South-Eastern Spain for potential anti-inflammatory effects targeting nuclear factor-Kappa B and other pro-inflammatory mediators
Journal of Ethnopharmacology, Vol.124(2), pp.295-305
2009
Metrics
19 Record Views
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Abstract
<p><h4 id="x-x-absSec_N37f832b8N373d50f8">Aim of the Study</h4> <p id="x-x-">Identification of plants with anti-inflammatory activity can be successfully based on information gained through knowledge on their traditional use. This is particularly true for biodiversity-rich regions of the world such as the Mediterranean. While such approaches are often single target based, here we used a multitarget, cell-based approach focusing on the pro-inflammatory signaling cascade and especially the NF-kappaB (NF-κB) pathway. <h4 id="x-x-absSec_N37f832b8N373d5158">Materials and methods</h4> <p id="x-x-">The plants from South-Eastern Spain were chosen on the basis that they were recorded as having a traditional use against an indication related to inflammation. The primary target was the transcription factor NF-κB (using a luciferase-based assay in HeLa cells). In addition extracts were tested <em>in vitro</em> for effects on cytokines (IL-6, IL-8, TNF-α) or PGE<sub>2</sub> in monocytes and for potential cytotoxic/pro-apoptotic action as well as for their influence on the cell cycle. <h4 id="x-x-absSec_N37f832b8N373d51e8">Results</h4> <p id="x-x-">Overall, 64 medicinal plant drugs from 61 species were assessed as potential inhibitors of inflammatory mediators to levels of 100–10 μg/ml. Three plants showed the highest level of activity (50 μg/ml) in inhibiting the activation of NF-κB in 5.1 cells: <em>Helichrysum stoechas</em> (Asteraceae), <em>Dorycnium pentaphyllum</em> (Fabaceae, s.l.) and <em>Phlomis almeriensis</em> (Lamiaceae). In the tests against the cytokines it was particularly striking to find that a number of species, <em>Bupleurum fruticosum</em>, <em>Chamaespartium tridentatum</em>, <em>Genista ramosissima</em>, <em>Helichrysum stoechas</em>, <em>Mercurialis tomentosa</em>, <em>Ononis ramosissima</em>, <em>Peganum harmala</em>, <em>Picnomon acarna</em>, <em>Retama sphaerocarpa</em> and <em>Santolina viscosa</em> showed extracts that were active at inhibiting TNF-α (10 μg/ml). <h4 id="x-x-absSec_N37f832b8N373d54b8">Conclusions</h4> <p id="x-x-"><em>Overall</em>, this project has identified a series of species with an activity profile which merits further phytochemical–pharmacological investigation.</p>
Details
- Title
- Assessing medicinal plants from South-Eastern Spain for potential anti-inflammatory effects targeting nuclear factor-Kappa B and other pro-inflammatory mediators
- Creators
- P Bremner - University of LondonD Rivera - University of MurciaM A Calzado - University of CordobaC Obón - University Miguel HernándezC Inocencio - University of MurciaC Beckwith - University of LondonB L Fiebich - University of FreiburgE Muñoz - University of CordobaM Heinrich - University of London
- Publication Details
- Journal of Ethnopharmacology, Vol.124(2), pp.295-305
- Identifiers
- 1259; 991012820786402368
- Academic Unit
- Southern Cross Plant Science
- Resource Type
- Journal article