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Published (Version of record)CC BY V4.0, Open Access
Published (Version of record)CC BY V4.0, Open
Journal article
Antimicrobial proteins from oyster hemolymph improve the efficacy of conventional antibiotics
PloS one, Vol.20(1), pp.1-28
21/01/2025
PMID: 39836702
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Source: InCites
Abstract
Discovering new antibiotics and increasing the efficacy of existing antibiotics are priorities to address antimicrobial resistance. Antimicrobial proteins and peptides (AMPPs) are considered among the most promising antibiotic alternatives and complementary therapies. Here, we build upon previous work investigating the antibacterial activity of a semi-purified hemo-lymph protein extract (HPE) of the Australian oyster Saccostrea glomerata. HPE showed antimicrobial-biofilm inhibitory activity toward laboratory and clinical strains of Streptococcus pneumoniae and Streptococcus pyogenes at 4.4 and 24.1 μg/mL total protein, respectively. In combination assays, the effectiveness of conventional antibiotics (ampicillin, gentamicin, trimethoprim and ciprofloxacin) was improved between 2 to 32-fold in the presence of HPE (1–12 μg/mL) against a range of clinically important bacteria including Streptococcus spp., Pseudomonas aeruginosa, Moraxella catarrhalis, Klebsiella pneumoniae and Staphylococcus aureus. Effective HPE concentrations are comparable to AMPPs currently approved for use or in clinical trials pipelines. Proteomics analysis of HPE identified a number of proteins including abundant known AMPPs. It was non-toxic to A549 human lung cells up to 205 μg/ mL, demonstrating safety well above effective concentrations. Activity was retained with storage at-80˚C and ambient laboratory temperature (~24˚C), but declined after treatment at either 37˚C or 60˚C (1 h). This study is in agreement with growing evidence that AMPPs show specificity and a high capacity for synergism with antibiotics. The discovery of HPE provides great opportunities for both pharmaceutical and aquaculture industry development.
Details
- Title
- Antimicrobial proteins from oyster hemolymph improve the efficacy of conventional antibiotics
- Creators
- Kate Summer - Southern Cross UniversityQi Guo - Southern Cross UniversityLei Liu - Southern Cross UniversitySarah Giles - Flinders UniversityBronwyn J Barkla - Southern Cross UniversityKirsten Benkendorff - Southern Cross University
- Publication Details
- PloS one, Vol.20(1), pp.1-28
- Publisher
- Public Library of Science
- Grant note
- Financial support was provided by the Australian Government Department of Education Research Training Program, the Faculty of Science and Engineering at Southern Cross University, and the Malacological Society of Australasia. Proteomics analysis was made possible by ARC LIEF funding (LE170100192).
- Identifiers
- 991013252561402368
- Copyright
- © 2025 Summer et al
- Academic Unit
- Research Infrastructure and Operations
- Language
- English
- Resource Type
- Journal article