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Published (Version of record)CC BY V4.0, Open Access
Published (Version of record)CC BY V4.0, Open
Journal article
Analgesic, anti-inflammatory and NF-κB inhibitory activity of aerial parts of Cestrum diurnum
Clinical Phytoscience, Vol.8, pp.1-9
07/03/2022
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Abstract
Background: Cestrum diurnum L. (Solanaceae), locally known as buno-Hasnahena, is widely used in different traditional medicinal practices to treat pain, burn, swelling and related disorders. Adequate evidence is not available to support its medicinal properties for further use and drug development. Present study was designed to evaluate its traditional use in pain and inflammation with further characterisation of its chemical constituents through liquid chromatography-mass spectroscopic (LC-MS) analysis.
Methods: Antinociceptive and analgesic potential of methanol extract of the aerial parts of C. diurnum was carried out using carrageenan induced paw oedema and formalin induced paw licking test in mice at the oral doses of 150 and 300 mg/kg body weight. Inhibition of the inflammatory mediator nuclear factor kappa B (NF-κB) was evaluated by tumour necrosis factor α (TNF-α) induced NF-κB activation assay in macrophage cells at the concentration of 100 μg/ml. LC-MS analysis of the extract was performed to characterise the active component responsible for bioactivities.
Results: The extract significantly inhibited (p < 0.05) carrageenan induced paw oedema at both doses tested and the effect persisted throughout the entire experimental period of 3 h with the highest activity (50% inhibition) observed at 3rd h. Further, the extract significantly inhibited (p < 0.05) formalin induced paw licking both in the early and late phase at the aforementioned dose levels. The extract also downregulated the expression of NF-κB p65 protein in the TNF-α induced NF-κB activation assay. LC-MS analysis of the extract indicated the presence of some important secondary metabolites including nicotine, nornicotine, ursolic acid, vitamin D3 and its derivatives.
Conclusions: The results of this study supported the folkloric uses of the plant in pain and inflammations. The insights and observations suggest the action might involve downregulation of NF-κB p65 protein expression and/or inhibition of autacoids (histamine, serotonin, prostaglandin).
Details
- Title
- Analgesic, anti-inflammatory and NF-κB inhibitory activity of aerial parts of Cestrum diurnum
- Creators
- Amina Khatun - Southern Cross UniversityMahmudur Rahman - Southern Cross UniversityMst. Luthfun Nesa - State University of BangladeshChung Yeng Looi - Taylor's UniversityWon Fen Wong - University of MalayaHazrina Hazni - University of MalayaMohamad Azrul bin Mahdzir - University of MalayaShaikh Jamal Uddin - Khulna UniversityKhalijah Awang - University of MalayaJamil A Shilpi - Khulna University
- Publication Details
- Clinical Phytoscience, Vol.8, pp.1-9
- Publisher
- SpringerOpen
- Identifiers
- 991013002594602368
- Copyright
- © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
- Academic Unit
- Science; Southern Cross Plant Science; Environmental Analysis Laboratory; Faculty of Science and Engineering
- Language
- English
- Resource Type
- Journal article