Journal article
An in vitro high-throughput assay for screening reproductive and toxic effects of anticancer compounds
Biotechnology and Applied Biochemistry, Vol.61(5), pp.582-592
2014
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Source: InCites
Abstract
<p>An <em>in vitro</em> assay was developed that simultaneously tested the effects of anticancer drug candidates on cytotoxicity, hormone synthesis, and gonadotrophin responsiveness using the choriocarcinoma JAr cell line. JAr culture conditions were optimized and then cells were exposed to a marine mollusc extract in the presence and absence of hCG. The intra- and interassay coefficients of variation of the optimized 1 H thiazolyl blue tetrazolium bromide assay were 11.3% and 10.9%, respectively. hCG (1,000 mIU/mL) increased progesterone (P<sub>4</sub>) synthesis after 24 H (<em>P</em> < 0.05). The mollusc extract significantly decreased cell viability, with the IC<sub>50</sub> affected by incubation time, but not hCG. P<sub>4</sub> synthesis was inhibited at low concentrations of the anticancer extract, but stimulated at the highest concentration, and complex interactions of P<sub>4</sub> were also found with hCG. In conclusion, the optimized assay is useful to characterize the effects of novel drugs on cytotoxicity, basal, and gonadotrophin-stimulated P<sub>4</sub> synthesis <em>in vitro</em>, and can be used to inform subsequent <em>in vivo</em> studies.</p>
Details
- Title
- An in vitro high-throughput assay for screening reproductive and toxic effects of anticancer compounds
- Creators
- Vicki Edwards - University of AdelaideKirsten Benkendorff - Southern Cross UniversityFiona Young - University of Adelaide
- Publication Details
- Biotechnology and Applied Biochemistry, Vol.61(5), pp.582-592
- Identifiers
- 3508; 991012821601202368
- Academic Unit
- School of Environment, Science and Engineering; Science; Faculty of Science and Engineering
- Resource Type
- Journal article