Journal article
An assessment of somatic embryogenesis and cryo-preservation methods with a wide range of Sitka spruce breeding material from the UK
Plant Cell, Tissue, and Organ Culture, Vol.131(3), pp.483-497
12/2017
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Source: InCites
Abstract
A wide range of Sitka spruce breeding material (Picea sitchensis (Bong.) Carr.) was assessed for its ability to respond to somatic embryogenesis (SE) and cryo-preservation methods, with 765 cell lines being established from seeds taken from the UK’s breeding population for testing with the relevant methodologies. Cell lines were most readily established from immature seeds (63%) on a proliferation medium originally developed for white spruce (P. glauca) with agar as the gelling agent. However, it was also found that 48% of the zygotic embryos taken from freshly harvested mature seed were also able to establish cell lines on the same medium, as were 20% of seeds that had been stored at −20 °C for up to 8 years. Most of the cell lines tested (57%) produced significant numbers of somatic embryos, but the rate at which they could be germinated into plants capable of surviving the transfer to nursery conditions varied considerably from 67 to 8% between years for reasons that were not clear. The ability of the cell lines to survive cryo-preservation was also assessed, and out of 199 cell lines tested, 45 cell lines from 21 breeding families were successfully established in a cryo-storage facility. The best rates of recovery were obtained when Phytagel™ was used in the medium for growing the samples before and after they were cryo-preserved.
Details
- Title
- An assessment of somatic embryogenesis and cryo-preservation methods with a wide range of Sitka spruce breeding material from the UK
- Creators
- Trevor Fenning - School of Environment, Science and Engineering Southern Cross University Lismore NSW AustraliaMargaret O’Donnell - Forest Research Northern Research Station Roslin Edinburgh EH25 9SY Midlothian Scotland, UKThomas Connolly - Forest Research Northern Research Station Roslin Edinburgh EH25 9SY Midlothian Scotland, UK
- Publication Details
- Plant Cell, Tissue, and Organ Culture, Vol.131(3), pp.483-497
- Publisher
- Springer Netherlands; Dordrecht
- Identifiers
- 991012927077902368
- Academic Unit
- Faculty of Science and Engineering; School of Environment, Science and Engineering
- Language
- English
- Resource Type
- Journal article