Journal article
A novel and high throughput approach to assess photosynthetic thermal tolerance of kelp using chlorophyll α fluorometry
Journal of phycology, 13296
07/11/2022
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Source: InCites
Abstract
Foundation seaweed species are experiencing widespread declines and localised extinctions due to increased instability of sea surface temperature. Characterising temperature thresholds are useful for predicting patterns of change and identifying species most vulnerable to extremes. Existing methods for characterising seaweed thermal tolerance produce diverse metrics and are often time consuming, making comparisons between species and techniques difficult, hindering insight into global patterns of change. Using three kelp species, we adapted a high throughput method - previously used in terrestrial plant thermal biology - for use on kelps. This method employs temperature-dependent fluorescence (T-F0) curves under heating or cooling regimes to determine the critical temperature (Tcrit) of photosystem II (PSII), i.e., the breakpoint between slow and fast rise fluorescence response to changing temperature, enabling rapid assays of photosynthetic thermal tolerance using a standardised metric. This method enables characterisation of Tcrit for up to 48 samples per two-hour assay, demonstrating the capacity of T-F0 curves for high-throughput assays of thermal tolerance. Temperature-dependent fluorescence curves and their derived metric, Tcrit, may offer a timely and powerful new method for the field of phycology, enabling characterisation and comparison of photosynthetic thermal tolerance of seaweeds across many populations, species, and biomes.
Details
- Title
- A novel and high throughput approach to assess photosynthetic thermal tolerance of kelp using chlorophyll α fluorometry
- Creators
- Rosalie J Harris - Australian National UniversityCallum Bryant - Australian National UniversityMelinda A Coleman - Southern Cross UniversityAndrea Leigh - University of Technology SydneyVerónica F Briceño - Australian National UniversityPieter A Arnold - Australian National UniversityAdrienne B Nicotra - Australian National University
- Publication Details
- Journal of phycology, 13296
- Comment
- We respectfully acknowledge the Yuin and Ngunnawal First Nations peoples as the Traditional Custodians of the lands where this research took place. We thank Peter Ralph, Executive Director of the C3 Research Institute, UTS, for invaluable early discuss ions on logistical considerations and the UTS Faculty of Science for technical support with the Diving PAM. Seaweed samples were collected under NSW Department of Primary Industries Scientific Collection Permit number: P01/0059(A)-3.0. The authors declare no conflict of interest.
- Publisher
- John Wiley & Sons, Ltd
- Grant note
- This research was supported by the Ecological Society of Australia through The Holsworth Wildlife Research Endowment, The Linnean Society of NSW through the Joyce W Vickery Scientific Research Fund and by the Australian Government Research Training Program, each awarded to R Harris.
- Identifiers
- 991013060891502368
- Copyright
- This article is protected by copyright. All rights reserved.
- Academic Unit
- Faculty of Science and Engineering
- Language
- English
- Resource Type
- Journal article