A novel and high-throughput approach to assess photosynthetic thermal tolerance of kelp using chlorophyll [alpha] fluorometry

Rosalie J Harris; Callum Bryant; Melinda A Coleman; Andrea Leigh; Verónica F Briceño; Pieter A Arnold; Adrienne B Nicotra

doi:10.1111/jpy.13296

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A novel and high-throughput approach to assess photosynthetic thermal tolerance of kelp using chlorophyll [alpha] fluorometry

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A novel and high-throughput approach to assess photosynthetic thermal tolerance of kelp using chlorophyll [alpha] fluorometry

Rosalie J Harris, Callum Bryant, Melinda A Coleman, Andrea Leigh, Verónica F Briceño, Pieter A Arnold and Adrienne B Nicotra

Journal of phycology, Vol.59(1), pp.179-192

02/2023

DOI: https://doi.org/10.1111/jpy.13296

Appears in Recent Faculty of Science and Engineering Publications

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Abstract

Analysis

Biomes

Chlorophyll

Fluorescence

Photosynthesis

Foundation seaweed species are experiencing widespread declines and localized extinctions due to increased instability of sea surface temperature. Characterizing temperature thresholds are useful for predicting patterns of change and identifying species most vulnerable to extremes. Existing methods for characterizing seaweed thermal tolerance produce diverse metrics and are often time-consuming, making comparisons between species and techniques difficult, hindering insight into global patterns of change. Using three kelp species, we adapted a high-throughput method – previously used in terrestrial plant thermal biology – for use on kelps. This method employs temperature-dependent fluorescence (T–F0) curves under heating or cooling regimes to determine the critical temperature (Tcrit) of photosystem II (PSII), i.e., the breakpoint between slow and fast rise fluorescence response to changing temperature, enabling rapid assays of photosynthetic thermal tolerance using a standardized metric. This method enables characterization of Tcrit for up to 48 samples per two-hour assay, demonstrating the capacity of T–F0 curves for high-throughput assays of thermal tolerance. Temperature-dependent fluorescence curves and their derived metric, Tcrit, may offer a timely and powerful new method for the field of phycology, enabling characterization and comparison of photosynthetic thermal tolerance of seaweeds across many populations, species, and biomes.

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Title: A novel and high-throughput approach to assess photosynthetic thermal tolerance of kelp using chlorophyll [alpha] fluorometry
Creators: Rosalie J Harris - Australian National University
Callum Bryant - Australian National University
Melinda A Coleman - Southern Cross University
Andrea Leigh - University of Technology Sydney
Verónica F Briceño - Australian National University
Pieter A Arnold - Australian National University
Adrienne B Nicotra - Australian National University
Publication Details: Journal of phycology, Vol.59(1), pp.179-192
Publisher: Wiley Subscription Services, Inc
Grant note: Australian Government Ecological Society of Australia Linnean Society of NSW
Identifiers: 991013089144302368
Copyright: © 2022 The Authors. Journal of Phycology published by Wiley Periodicals LLC on behalf of Phycological Society of America. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Academic Unit: Faculty of Science and Engineering
Language: English
Resource Type: Journal article

A novel and high-throughput approach to assess photosynthetic thermal tolerance of kelp using chlorophyll [alpha] fluorometry

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