Journal article
A FRUITFULL-like gene is associated with genetic variation for fruit flesh firmness in apple (Malus domestica Borkh.)
Tree Genetics & Genomes, Vol.6(2), pp.271-279
2010
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Source: InCites
Abstract
<p>The <em>FRUITFULL</em> (<em>FUL</em>) and <em>SHATTERPROOF</em> (<em>SHP</em>) genes are involved in regulating fruit development and dehiscence in <em>Arabidopsis</em>. We tested the hypothesis that this class of genes are also involved in regulating the development of fleshy fruits, by exploring genetic and phenotypic variation within the apple (<em>Malus domestica</em>) gene pool. We isolated and characterised the genomic sequences of two candidate orthologous <em>FUL</em>-like genes, <em>MdMADS2.1</em> and <em>MdMADS2.2</em>. These were mapped using the reference population ‘Prima x Fiesta’ to loci on <em>Malus</em> linkage groups LG14 and LG06, respectively. An additional MADS-box gene, <em>MdMADS14</em>, shares high amino acid identity with the <em>Arabidopsis SHATTERPROOF1/2</em> genes and was mapped to <em>Malus</em> linkage group LG09. Association analysis between quantitative fruit flesh firmness estimates of ‘Prima x Fiesta’ progeny and the <em>MdMADS2.1</em>, <em>MdMADS2.2</em> and <em>MdMADS14</em> loci was carried out using a mixed model analysis of variance. This revealed a significant association (<em>P</em> < 0.01) between <em>MdMADS2.1</em> and fruit flesh firmness. Further evidence for the association between <em>MdMADS2.1</em> and fruit flesh firmness was obtained using a case–control population-based genetic association approach. For this, a polymorphic repeat, (AT)<sub>n</sub>, in the 3′ UTR of <em>MdMADS2.1</em> was used as a locus-specific marker to screen 168 apple accessions for which historical assessments of fruit texture attributes were available. This analysis revealed a significant association between the <em>MdMADS2.1</em> and fruit flesh firmness at both allelic (<em>χ</em> <sup>2</sup> = 34, <em>df</em> = 9, <em>P</em> < 0.001) and genotypic (<em>χ</em> <sup>2</sup> = 57, <em>df</em> = 32, <em>P</em> < 0.01) levels.</p>
Details
- Title
- A FRUITFULL-like gene is associated with genetic variation for fruit flesh firmness in apple (Malus domestica Borkh.)
- Creators
- Volkan Cevik - Warwick UniversityCarol D Ryder - Warwick UniversityAlexander Popovich - Warwick UniversityKenneth Manning - Warwick UniversityGraham J King - Rothamsted ResearchGraham B Seymour - University of Nottingham
- Publication Details
- Tree Genetics & Genomes, Vol.6(2), pp.271-279
- Identifiers
- 1189; 991012820567202368
- Academic Unit
- Faculty of Science and Engineering; Science; Southern Cross Plant Science
- Resource Type
- Journal article