Analysis of rice grain protein composition in the context of rice grain quality

Protein (composed of prolamin, glutelin, globulin and albumin) is the second most abundant component in the milled rice grain. It has very diverse properties that may contribute to rice grain quality. Rice grain protein extraction and high-performance liquid chromatography methods were developed and applied into different sets of short, medium and long grain advanced breeding lines. Rice prolamin and albumin were extracted with 60% n-propanol, while the glutelin and globulin were extracted with 5M acetic acid. Analyses of protein extracts revealed differences in rice grain storage protein composition which were associated with rice grain texture and head rice yield.