Raw spectral data - SWATH-MS of Cannabis sativa glandular trichomes in response to abscisic acid and drought stress

Nicolas Dimopoulos; Qi Guo; Ben Liu; Matt Nolan; Lennard Garcia-de Heer; Jos C Mieog; Bronwyn J Barkla; Tobias Kretzschmar

doi:10.25918/data.431

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Raw spectral data - SWATH-MS of Cannabis sativa glandular trichomes in response to abscisic acid and drought stress

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Raw spectral data - SWATH-MS of Cannabis sativa glandular trichomes in response to abscisic acid and drought stress

Nicolas Dimopoulos, Qi Guo, Ben Liu, Matt Nolan, Lennard Garcia-de Heer, Jos C Mieog, Bronwyn J Barkla and Tobias Kretzschmar

Southern Cross University

03/2025

DOI:

https://doi.org/10.25918/data.431

Appears in Recent Faculty of Science and Engineering Publications

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Abstract

Cannabinoids

terpenoids

Cannabis sativa

drought

water deficit

trichome

primary metabolism

secondary metabolism

abscisic acid

salicylic acid

ethylene

SWATH-MS

Plant biology

Plant physiology

Cannabis (Cannabis sativa L.) is a medicinally important drought resistant plant. The glandular trichomes (GT) on female flowers of cannabis are the main site of production and storage of cannabinoids and terpenoids. While water deficit stress (WD) modulates cannabinoid content in cannabis, it is unknown how GT regulation is affected to achieve this. We quantified the GT proteome and floral cannabinoid and terpenoid content in late flowering ‘Hindu Kush’ cannabis treated with either short-term WD or with an abscisic acid (ABA) foliar spray. We found that WD and ABA changed the abundance of many of the same proteins in the GT, with changes reflecting a decrease in primary metabolic processes and an increase in stress response mechanisms. Results suggested ABA likely mediates changes in the GT indirectly by affecting salicylic acid and ethylene signalling. Interestingly, WD caused cannabinoid content to increase, while ABA caused monoterpene content to increase, and proteins involved in cannabinoid or terpenoid biosynthesis were either downregulated or did not change in either treatment. Since photosynthesis and the total photoassimilate pool typically decline in response to WD in plants, we postulate that the remaining photoassimilate pool is reallocated to support secondary metabolism in the Cannabis GT when responding to WD. This dataset release contains the associated SWATH-MS raw spectral data for protein abundance in glandular trichomes. Also included are the floral cannabinoid, terpenoid and biomass values.

Details

Title: Raw spectral data - SWATH-MS of Cannabis sativa glandular trichomes in response to abscisic acid and drought stress
Creators: Nicolas Dimopoulos (Author) - Southern Cross University
Qi Guo (Author) - Southern Cross University
Ben Liu (Author) - Southern Cross University
Matt Nolan (Author) - Southern Cross University
Lennard Garcia-de Heer (Author) - Southern Cross University
Jos C Mieog (Author) - Southern Cross University
Bronwyn J Barkla (Author) - Southern Cross University
Tobias Kretzschmar (Corresponding Author) - Southern Cross University
Publisher: Southern Cross University
Identifiers: 991013240313402368
Academic Unit: Faculty of Science and Engineering
Language: English
Resource Type: Dataset

Raw spectral data - SWATH-MS of Cannabis sativa glandular trichomes in response to abscisic acid and drought stress

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