Incubation chamber volume and algal biomass, surface area and epiphyte biomass of Fucus vesiculosus specimen sampled at approx 1.5 m depth in the coastal Baltic Sea at Tvärminne Zoological Station, Hanko, Finland, in August 2020
Hagen Buck-Wiese, Mona A Andskog, Nguyen P Nguyen, Margot Bligh, Camilla Gustafsson, Eero Asmala, Manuel Liebeke, Silvia Vidal-Melgosa and Jan-Hendrik Hehemann
carbon dioxide removal carbon sequestration Chamber number Chamber volume Contributions of organic carbon from vegetated coastal ecosystems (OC-VCE) Dissolved Organic Carbon Epiphytes, biomass as dry weight fucose-containing sulfated polysaccharides Fucus vesiculosus Fucus vesiculosus, biomass as dry weight Fucus vesiculosus, leaf area Macroalgae marine glycans Treatment: light condition
For the algae incubations on August 18, 2020, transparent gas impermeable plastic vacuum bags (Packing24, PA/PE 20/70, 90my) with a single F. vesiculosus thallus, were closed with rubber bands around PVC end cap fitting of 12 cm diameter and attached to a bottom-anchored line at ~1 m depth. Thalli of ~50 cm length were detached close to the base by cutting the few millimeters thick, old stipe. Half of the incubations were covered with black bags to simulate dark conditions. Immediately prior to starting the incubations, water was sampled from the ambient water to determine initial conditions. At the end of the incubation, all algal tissue was collected. Water from within chambers was collected for analyses and the total volume measured. In the laboratory, the algal thallus was spread out across a cutting mat with a grid for surface area estimation. Epiphytes and epifauna were scraped off thalli using a razor blade, and collected separately from the algal biomass. All biomass was frozen and subsequently freeze-dried for 24 hours, and weighed to determine dry weight.
Incubation chamber volume and algal biomass, surface area and epiphyte biomass of Fucus vesiculosus specimen sampled at approx 1.5 m depth in the coastal Baltic Sea at Tvärminne Zoological Station, Hanko, Finland, in August 2020
Creators
Hagen Buck-Wiese
Mona A Andskog
Nguyen P Nguyen
Margot Bligh
Camilla Gustafsson
Eero Asmala
Manuel Liebeke
Silvia Vidal-Melgosa
Jan-Hendrik Hehemann
Publisher
PANGAEA
Grant note
DFG Emmy Noether program - HE7217/1-1 / Deutsche Forschungsgemeinschaft, Bonn (https://doi.org/10.13039/501100001659)
EXC 2077: The Ocean Floor – Earth's Uncharted Interface - 390741603 / German Research Foundation (https://doi.org/10.13039/501100001659)
DFG Heisenberg program - HE7217/5-1 / Deutsche Forschungsgemeinschaft, Bonn (https://doi.org/10.13039/501100001659)