Establishing an open-source platform for unravelling the genetics of macadamia: integration of linkage and genome maps.
We have generated an anchored, chromosome-scale genome assembly for M. integrifolia cultivar HAES 741 (4,094 scaffolds, 745 Mb, N50 413 kb) using a combination of high coverage Illumina short and PacBio long read sequences. Scaffolds were anchored to pseudo-chromosomes using seven genetic linkage maps derived from progeny with HAES 741 parentage. This assembly has improved contiguity and coverage, with >120 Gb of new sequence in comparison to the first draft (Nock et al. 2016). Following annotation, a repeat content of 55% and 34,274 protein-coding genes were predicted.
A full description of the process for generating the data is provided in the manuscript “Chromosome-scale assembly and annotation of the macadamia genome”