Conference presentation
Dynamics of nasopharyngeal colonisation & correlations with lymphocyte populations in the adenoid & peripheral blood of chronic otitis media prone children
International Symposium on Recent Advances in Otitis Media, 19th (Gold Coast, Queensland, 04/06/2017 - 08/06/2017)
2018
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Abstract
<p>Aim: Nasopharyngeal cultures of chronic otitis media (COM)-prone and non COM-prone children were correlated with blood and adenoid lymphocyte populations to evaluate microbe-host relationships relating to the pathogenesis of COM. The clinical significance of nasopharyngeal aspirate (NPA) culture as a screening tool for adenoid culture was also assessed. <br /><br />Methodology: Forty children scheduled for adenoidectomy were enrolled into COM-prone or non COM-prone groups (for each, <em>n</em> = 20). Conventional culture was performed for adenoid biopsy and NPA bacteriology. Peripheral blood and adenoid mononuclear cells (PBMC and AdMNC, respectively) were stained with viability stain, monoclonal anti-CD19, anti-CD3, anti-CD4, anti-CD8, anti-CD25 and anti-CD127. Cells were permeabilised, fixed and intracellularly stained with monoclonal anti-FoxP3 and quantified by flow cytometry.<br /><br />Results: <em>Streptococcus pneumoniae</em>, <em>Staphylococcus aureus</em>, <em>Moraxella catarrhalis</em>and non-typeable <em>Haemophilus influenzae</em> (NTHi) were positive in 44%, 39%, 22% and 22% of COM-prone children with no significant differences compared to non COM-prone children. <em>S. pneumoniae</em> NPA culture was a significant predictor of <em>S. pneumoniae</em> culture at the adenoid (OR = 39.30, CI = 3.68 – 419.28, <em>p</em> = 0.002). Children with positive otopathogen nasopharyngeal culture had significantly more FoxP3+ CD25hi+ CD127lo+ PBMC (<em>M</em> = 4.4%) compared to otopathogen culture negative children (<em>M</em> = 3.1%, <em>p</em> = 0.005).<br /> <br />Conclusion: Otopathogens are positively associated with regulatory T cells, potentially inducing a suppressive effector response to promote colonisation and infection chronicity, thus supporting further investigation of their influence on lymphocyte activity. <em>S. pneumoniae</em> NPA cultures may be of direct clinical benefit to clinicians in making diagnoses and recommendations for prophylactic antibiotic therapies in COM.</p>
Details
- Title
- Dynamics of nasopharyngeal colonisation & correlations with lymphocyte populations in the adenoid & peripheral blood of chronic otitis media prone children
- Creators
- Jessica J Browne - Central Queensland UniversityEvan H Matthews - Central Queensland UniversityAndrew W Taylor-Robinson - Central Queensland UniversityJenelle M Kyd - Central Queensland University
- Conference
- International Symposium on Recent Advances in Otitis Media, 19th (Gold Coast, Queensland, 04/06/2017 - 08/06/2017)
- Identifiers
- 3875; 991012822187702368
- Academic Unit
- Faculty of Health; School of Health and Human Sciences; Human Sciences
- Resource Type
- Conference presentation