Book chapter
Growing arabidopsis in vitro: cell suspensions, in vitro culture, and regeneration
Arabidopsis protocols, Vol.1062, pp.53-62
Methods in molecular biology, v. 1062, Humana Press/Springer, Third edition
2014
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Abstract
An understanding of basic methods in Arabidopsis tissue culture is beneficial for any laboratory working on this model plant. Tissue culture refers to the aseptic growth of cells, organs, or plants in a controlled environment, in which physical, nutrient, and hormonal conditions can all be easily manipulated and monitored. The methodology facilitates the production of a large number of plants that are genetically identical over a relatively short growth period. Techniques, including callus production, cell suspension cultures, and plant regeneration, are all indispensable tools for the study of cellular biochemical and molecular processes. Plant regeneration is a key technology for successful stable plant transformation, while cell suspension cultures can be exploited for metabolite profiling and mining. In this chapter we report methods for the successful and highly efficient in vitro regeneration of plants and production of stable cell suspension lines from leaf explants of both Arabidopsis thaliana and Arabidopsis halleri.
Details
- Title
- Growing arabidopsis in vitro: cell suspensions, in vitro culture, and regeneration
- Creators
- Bronwyn J Barkla - Instituto de Biotecnología/UNAMRosario Vera-Estrella - Instituto de Biotecnología/UNAMOmar Pantoja - Instituto de Biotecnología/UNAM
- Contributors
- Jose J Sánchez-Serrano (Editor of compilation)Julio Salinas (Editor of compilation)
- Publication Details
- Arabidopsis protocols, Vol.1062, pp.53-62
- Series
- Methods in molecular biology; v. 1062
- Publisher
- Humana Press/Springer; New York, NY
- Edition
- Third edition
- Identifiers
- 1747; 991012821287402368
- Copyright
- © 2014 Springer Science+Business Media New York.
- Academic Unit
- Faculty of Science and Engineering; Science; Southern Cross Plant Science
- Language
- English
- Resource Type
- Book chapter