Journal article
Multidrug resistant Aeromonas spp. isolated from zebrafish (Danio rerio): antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes
Letters in Applied Microbiology, Vol.68(5), pp.370-377
2019
PMID: 30790321
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Abstract
Aeromonas spp. are Gram‐negative opportunistic bacteria which have been commonly associated with fish diseases. In this study, antibiogram, antimicrobial resistance genes and integrons of 43 zebrafish‐borne Aeromonas spp. were studied. The isolates were identified as six Aeromonas species (A. veronii biovar veronii (n = 26), A. veronii biovar sobria (n = 3), A. hydrophila (n = 8), A. caviae (n = 3), A. enteropelogenes (n = 2) and A. dhakensis (n = 1)). Antibiogram of the isolates indicated that most of them were resistant to amoxicillin (100·00%), nalidixic acid (100·00%), oxytetracycline (100·00%), ampicillin (93·02%), tetracycline (74·42%), rifampicin (67·44%) and imipenem (65·15%). Multiple antimicrobial resistance (MAR) index values ranged from 0·19–0·44 to 90·70% isolates showed multidrug resistance. PCR of antimicrobial resistance genes revealed that the tetracycline resistance gene (tetA) was the most predominant (67·44%) among the isolates. The qnrS (53·49%), tetB (30·23%), tetE (30·23%), qnrB (23·26%) and aac(6’)‐Ib‐cr (4·65%) genes were also detected. Class 1 integrase (IntI1) gene was found in 46·51% of the isolates. Two types of class 1 integron gene cassette profiles (qacG‐aadA6‐qacG and drfA1) were identified. The results showed that zebrafish‐borne aeromonads can harbour different types of antimicrobial resistance genes and class 1 integrons.
Significance and Impact of the Study
Aeromonas spp. are important pathogens found in diverse environments. Antimicrobial resistance genes and integrons of ornamental fish‐borne Aeromonas spp. are not well studied. The antibiogram, antimicrobial resistance genes and class 1 integrons of Aeromonas spp. isolated from zebrafish were characterized for the first time in Korea. The prevalence of tetracycline resistance genes, plasmid‐mediated quinolone resistance genes and class 1 integron gene cassettes were observed among the isolates. The qacG‐aadA6‐qacG gene cassette was identified for the first time in Aeromonas spp. The results suggest that the wise use of antimicrobials is necessary for the better management of the ornamental fish.
Details
- Title
- Multidrug resistant Aeromonas spp. isolated from zebrafish (Danio rerio): antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes
- Creators
- S Hossain - Chungbuk National UniversityP S Dahanayake - Chungbuk National UniversityB C J De Silva - Southern Cross University, Southern Cross Analytical Research ServicesM V K S Wickramanayake - Chungbuk National UniversityS H M P Wimalasena - Chungbuk National UniversityG-J Heo - Chungbuk National University
- Publication Details
- Letters in Applied Microbiology, Vol.68(5), pp.370-377
- Publisher
- Society for Applied Microbiology
- Identifiers
- 991012900099502368
- Academic Unit
- Southern Cross Analytical Research Services; Faculty of Science and Engineering; School of Environment, Science and Engineering
- Language
- English
- Resource Type
- Journal article